Chitinase-3-like protein 1, a 40 KDa secreted and Glycosylated extracellular matrix protein
that plays
an important role in the processes of inflammation and tissue remodeling. It is expressed
in articular chondrocytes, synovial cells as well as in liver. However, CHI3L1 is not synthesized under
state of health, an induction of this protein expression is observed in the patients with rheumatoid
arthritis and cancer. It was over-expressed in the one of the most over-expressed genes in glioblastoma,
papillary
thyroid carcinoma, and extracellular myxoid chondrosarcoma. It is also upregulated in a number
of solid tumors, such as cancer of the breast, colon, lung, kidney, and ovary. In cancer patients, serum
CHI3L1 concentrations
are often predictive of tumor stages, response to therapy, and prognosis.
Chitinase-3-like protein 1 also is an inflammatory biomarker, which can be upregulated after pro-inflammatory
cytokine stimulation, possesses an ability to enhance the adhesion and internalization of intracellular bacteria
into colonic epithelial cells. CHI3L1 is upregulated in inflammatory conditions, including both acute and chronic
colitis. Neutralization of CHI3L1 by anti-CHI3L1 Ab significantly suppresses DSS-induced acute colitis.
| Amino Acid Sequence of Human Chitinase-3-like protein 1 : 383 aa, MW 42625 Da, Cellular component: Secreted, extracellular space; |
| Molecular function:May play an important role in the capacity of cells to respond to and cope with changes in their environment. May play a role in tissue remodeling and defense against pathogens. ; Swiss ID: P36222 |
10 20 30 40 50 60
MGVKASQTGF VVLVLLQCCS AYKLVCYYTS WSQYREGDGS CFPDALDRFL CTHIIYSFAN
70 80 90 100 110 120
ISNDHIDTWE WNDVTLYGML NTLKNRNPNL KTLLSVGGWN FGSQRFSKIA SNTQSRRTFI
130 140 150 160 170 180
KSVPPFLRTH GFDGLDLAWL YPGRRDKQHF TTLIKEMKAE FIKEAQPGKK QLLLSAALSA
190 200 210 220 230 240
GKVTIDSSYD IAKISQHLDF ISIMTYDFHG AWRGTTGHHS PLFRGQEDAS PDRFSNTDYA
250 260 270 280 290 300
VGYMLRLGAP ASKLVMGIPT FGRSFTLASS ETGVGAPISG PGIPGRFTKE AGTLAYYEIC
310 320 330 340 350 360
DFLRGATVHR ILGQQVPYAT KGNQWVGYDD QESVKSKVQY LKDRQLAGAM VWALDLDDFQ
370 380
GSFCGQDLRF PLTNAIKDAL AAT 383 |
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| (Keio J Med 56 (1) : 21－27, March 2007) |
| Professional Custom Sample Assay |
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| Western blot analysis of GP-39 (CHI3L1) expression in postnatal mouse liver tissue extract by using PS001-31-AG-03-50. |
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Western blot analysis of GP-39 (CHI3L1) expression in rat liver tissue extract by using PS001-31-AG-04-50. |
| The chitinase 3-like protein human cartilage glycoprotein 39 inhibits cellular responses to the inflammatory cytokines interleukin-1 and tumour necrosis factor-alpha |
Expression of the chitinase 3-like protein HC-gp39 (human cartilage glycoprotein 39) is associated with conditions of increased matrix turnover and tissue remodelling. High levels of this protein have been found in sera and synovial fluids of patients with inflammatory and degenerative arthritis. In order to assess the role of HC-gp39 in matrix degradation induced by inflammatory cytokines, we have examined its effect on the responses of connective tissue cells to TNF-alpha (tumour necrosis factor-alpha) and IL-1 (interleukin-1) with respect to activation of signalling pathways and production of MMPs (matrix metalloproteases) and chemokines. Stimulation of human skin fibroblasts or articular chondrocytes with IL-1 or TNF-alpha in the presence of HC-gp39 resulted in a marked reduction of both p38 mitogen-activated protein kinase and stress-activated protein kinase/Jun N-terminal kinase phosphorylation, whereas nuclear translocation of nuclear factor kappaB proceeded unimpeded. HC-gp39 suppressed the cytokine-induced secretion of MMP1, MMP3 and MMP13, as well as secretion of the chemokine IL-8. The suppressive effects of HC-gp39 were dependent on phosphoinositide 3-kinase activity, and treatment of cells with HC-gp39 resulted in AKT-mediated serine/threonine phosphorylation of apoptosis signal-regulating kinase 1. This process could therefore be responsible for the down-regulation of cytokine signalling by HC-gp39. These results suggest a physiological role for HC-gp39 in limiting the catabolic effects of inflammatory cytokines. |
Hua Ling and Anneliese D Recklies. Biochem J. 2004 June 15; 380(Pt 3): 651–659. |
| Role of mammalian chitinases in inflammatory conditions |
Abstract. It has been hypothesized that dysregulated host/microbial interactions play a pivotal role in the pathogenesis of inflammatory bowel disease. However, the exact mechanisms underlying the induction and perpetuation of the intestinal disorder are unclear. Recently, we unexpectedly discovered significantly upregulated gene expression of chitinase 3-like-1 in inflamed colon of the dextran sulfate sodium-induced colitis model by employing the DNA-microarray analysis. Chitinase 3-like-1 has a chitin binding ability, but lacks the enzymatic activity of lysing microbial cell wall. Chitinase 3-like-1 protein is mainly expressed in colonic epithelial cells and macrophages in the inflamed colon of dextran sulfate sodium-induced colitis. Chitinase 3-like-1, which can be upregulated after pro-inflammatory cytokine stimulation, possesses an ability to enhance the adhesion and internalization of intracellular bacteria into colonic epithelial cells. Most importantly, in vivo neutralization of chitinase 3-like-1 significantly suppressed the development of dextran sulfate sodium-induced colitis by dramatically decreasing the bacterial adhesion and invasion into colonic epithelial cells. Furthermore, anti-chitinase 3-like-1 antibody-treated mice exhibited a significantly lower load of Salmonella typhimurium in peripheral organs as compared to control rabbit IgG-treated mice. Recently, it has been reported that acidic mammalian chitinase is expressed in the setting of T helper-2-associated inflammation and subsequently induces airway hyperresponsiveness in allergic asthma patients. In addition, pan-chitinase inhibitor significantly ameliorates T helper-2-mediated inflammation and airway hypersensitivity. These studies provide to be a novel insight into the physiological role of mammalian chitinases in host/microbial interactions, and inhibition of chitinase activity would be considered a novel therapeutic strategy of allergic and inflammatory disorders. (Keio J Med 56 (1) : 21－27, March 2007) |
Custom Service |
Sample vol. | Dilution | Range | Sample type | Catalog No. |
Quantity
|
Price ($) |
|---|---|---|---|---|---|---|---|
| Human Chitinase-3-like protein 1 Assay | 50 ul |
50 x |
62.5 -2000 pg/ml | Serum or EDTA plasma, cell culture supernates |
PS001-31-SH |
40
|
40 |
| Mouse Chitinase-3-like protein 1 Assay | 50 ul |
50 x |
62.5 -2000 pg/ml |
Serum or EDTA plasma, cell culture supernates |
PS001-31-SM |
40
|
40 |
| Antibody Name | Application | Species | Host | Catalog No | Quantity |
Price ($) |
| Chitinase-3-like protein 1 Antibody | WB, E | human | goat | PS001-31-AG-01-50 | 50 ug |
350 |
| Chitinase-3-like protein 1 Monoclonal Antibody | WB, IHC, E | human | rat | PS001-31-MAG-01-50 | 50 ug |
240 |
| Chitinase-3-like protein 1 Antibody | WB | h, r, m | goat | PS001-31-AG-04-50 | 50 ug |
160 |
| Chitinase-3-like protein 1 Antibody | WB | mouse | goat | PS001-31-AG-02-50 | 50 ug |
350 |
| Chitinase-3-like protein 1 Monoclonal Antibody | WB, E | mouse | rat | PS001-31-MAG-02-50 | 50 ug |
240 |
| Chitinase-3-like protein 1 C-Terminal Antibody | WB, E | mouse | goat | PS001-31-AG-03-50 | 50 ug |
160 |