| Amino Acid Sequence of Human Fos Related Antigen-1: 271 aa, MW 29413 Da, Cellular component: nucleus |
| Total aa: 271 ; MW : 29413 Da; Cellular component: nucleus; Molecular function: transcription factor activity and positive regulation of cell proliferation; Swiss ID: P15407 |
10 20 30 40 50 60
MFRDFGEPGP SSGNGGGYGG PAQPPAAAQA AQQKFHLVPS INTMSGSQEL QWMVQPHFLG
70 80 90 100 110 120
PSSYPRPLTY PQYSPPQPRP GVIRALGPPP GVRRRPCEQI SPEEEERRRV RRERNKLAAA
130 140 150 160 170 180
KCRNRRKELT DFLQAETDKL EDEKSGLQRE IEELQKQKER LELVLEAHRP ICKIPEGAKE
190 200 210 220 230 240
GDTGSTSGTS SPPAPCRPVP CISLSPGPVL EPEALHTPTL MTTPSLTPFT PSLVFTYPST
250 260 270
PEPCASAHRK SSSSSGDPSS DPLGSPTLLA L 271 |
| Amino Acid Sequence of Human Fos Related Antigen-2 |
Total aa: 326; MW: 35193 Da; Cellular component: nucleus; Molecular function: Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation; Swiss ID: P15408 |
10 20 30 40 50 60
MYQDYPGNFD TSSRGSSGSP AHAESYSSGG GGQQKFRVDM PGSGSAFIPT INAITTSQDL
70 80 90 100 110 120
QWMVQPTVIT SMSNPYPRSH PYSPLPGLAS VPGHMALPRP GVIKTIGTTV GRRRRDEQLS
130 140 150 160 170 180
PEEEEKRRIR RERNKLAAAK CRNRRRELTE KLQAETEELE EEKSGLQKEI AELQKEKEKL
190 200 210 220 230 240
EFMLVAHGPV CKISPEERRS PPAPGLQPMR SGGGSVGAVV VKQEPLEEDS PSSSSAGLDK
250 260 270 280 290 300
AQRSVIKPIS IAGGFYGEEP LHTPIVVTST PAVTPGTSNL VFTYPSVLEQ ESPASPSESC
310 320
SKAHRRSSSS GDQSSDSLNS PTLLAL 326 |
   |
| Immunostaining of Fos Related Antigen-1 by Rabbit Anti-Mouse Fra-1, N-Terminal Antibody |
 |
| human cervical cancer was stained nuclear of tumor cells by Fra-1 monoclonal antibody |
 |
| Representative PR and Fra2 immunostainings and analysis of PR and Fra2 expression in the DLP. Representative immune stainings of PR (A-C) and Fra2 (E-G) from Cast, Cast+E2 and Cast+E2+Fis 30 groups. Magnifications: 40x. JPET Fast Forward. Published on June 26, 2008 as DOI:10.1124/jpet.108.139501 |
  |
| Prostate carcinoma stained with Rabbit Anti-Human Fos Related Antigen-2, N-Terminal Antibody. 40KDa of Fra-2 in WB. |
CREB-1 and AP-1 transcription factors JunD and Fra-2 regulate bone sialoprotein gene expression in human breast cancer cell |
| Bone sialoprotein (BSP) expression is detected in a variety of human osteotropic cancers. High expression of BSP in breast and prostate primary carcinomas is associated with progression and bone metastases development. In this study, we examined the transcriptional regulation of BSP gene expression in MDA-MB-231 and MCF-7 human breast cancer cells compared with Saos-2 human osteoblast-like cells. BSP human promoter deletion analyses delineated a -56/-84 region, which comprises a cAMP response element (CRE) that was sufficient for maximal promoter activity in breast cancer cell lines. We found that the basic fibroblast growth factor response element (FRE) also located in the proximal promoter was a crucial regulator of human BSP promoter activity in Saos-2 but not in breast cancer cells. Promoter activity experiments in combination with DNA mobility shift assays demonstrated that BSP promoter activity is under the control of the CRE element, through CREB-1, JunD and Fra-2 binding, in MDA-MB-231, MCF-7 and in Saos-2 cells. Forskolin, a protein kinase A pathway activator, failed to enhance BSP transcriptional activity suggesting that CRE site behaves as a constitutive rather than an inducible element in these cell lines. Over-expression of JunD and Fra-2 increased BSP promoter activity and upregulated endogenous BSP protein expression in MCF-7 and Saos-2 cells while siRNA-mediated inhibition of both factors expression significantly reduced BSP protein level in MDA-MB-231. Collectively, these data provide with new transcriptional mechanisms, implicating CREB and AP-1 factors, that control BSP gene expression in breast cancer cells. |
| Detry C, et al. Bone. 2008 Feb;42(2):422-31. Epub 2007 Nov 1. |
|
Name |
Application | Catalog No. |
Quantity |
Price ($) |
|---|---|---|---|---|
| Human Fos Related Antigen-1 (Fra-1), C-Terminal, Monoclonal Antibody | WB, IHC, |
PS001-27--MAB-01 |
200 ul |
190 |
| Mouse Fos Related Antigen-1(Fra-1) , N-Terminal Antibody | IHC, IFC, WB |
PS001-27-AG-01 |
200 ul |
190 |
| Human Fos Related Antigen-2 (Fra-2), N-terminal, Antibody | IHC, IFC,WB, ChIP |
PS001-28-AG-01 |
200 ul |
190 |