IQGAP3, Phoenix Secretomics

Ras GTPase-activating-like protein IQGAP1, -2 & -3

IQGAP3: total 1631 amino acids, MW 184547 Da
MERRAAGPGW AAYERLTAEE MDEQRRQNVA YQYLCRLEEA KRWMEACLKE ELPSPVELEE SLRNGVLLAK LGHCFAPSVV PLKKIYDVEQ LRYQATGLHF RHTDNINFWL SAIAHIGLPS TFFPETTDIY DKKNMPRVVY CIHALSLFLF RLGLAPQIHD LYGKVKFTAE ELSNMASELA KYGLQLPAFS KIGGILANEL SVDEAAVHAA VLAINEAVER GVVEDTLAAL QNPSALLENL REPLAAVYQE MLAQAKMEKA ANARNHDDRE SQDIYDHYLT QAEIQGNINH VNVHGALEVV DDALERQSPE ALLKALQDPA LALRGVRRDF ADWYLEQLNS DREQKAQELG LVELLEKEEV QAGVAAANTK GDQEQAMLHA VQRINKAIRR GVAADTVKEL MCPEAQLPPV YPVASSMYQL ELAVLQQQQG ELGQEELFVA VEMLSAVVLI NRALEARDAS GFWSSLVNPA TGLAEVEGEN AQRYFDALLK LRQERGMGED FLSWNDLQAT VSQVNAQTQE ETDRVLAVSL INEALDKGSP EKTLSALLLP AAGLDDVSLP VAPRYHLLLV AAKRQKAQVT GDPGAVLWLE EIRQGVVRAN QDTNTAQRMA LGVAAINQAI KEGKAAQTER VLRNPAVALR GVVPDCANGY QRALESAMAK KQCPADTAFW VQHDMKDGTA YYFHLQTFQG IWEQPPGCPL NTSHLTREEI QSAVTKVTAA YDRQQLWKAN VGFVIQLQAR LRGFLVRQKF AEHSHFLRTW LPAVIKIQAH WRGYRQRKIY LEWLQYFKAN LDAIIKIQAW ARMWAARRQY LRRLHYFQKN VNSIVKIQAF FRARKAQDDY RILVHAPHPP LSVVRRFAHL LNQSQQDFLA EAELLKLQEE VVRKIRSNQQ LEQDLNIMDI KIGLLVKNRI TLQEVVSHCK KLTKRNKEQL SDMMVLDKQK GLKSLSKEKR QKLEAYQHLF YLLQTQPIYL AKLIFQMPQN KTTKFMEAVI FSLYNYASSR REAYLLLQLF KTALQEEIKS KVEQPQDVVT GNPTVVRLVV RFYRNGRGQS ALQEILGKVI QDVLEDKVLS VHTDPVHLYK NWINQTEAQT GQRSHLPYDV TPEQALSHPE VQRRLDIALR NLLAMTDKFL LAITSSVDQI PYGMRYVAKV LKATLAEKFP DATDSEVYKV VGNLLYYRFL NPAVVAPDAF DIVAMAAGGA LAAPQRHALG AVAQLLQHAA AGKAFSGQSQ HLRVLNDYLE ETHLKFRKFI HRACQVPEPE ERFAVDEYSD MVAVAKPMVY ITVGELVNTH RLLLEHQDCI APDHQDPLHE LLEDLGELPT IPDLIGESIA ADGHTDLSKL EVSLTLTNKF EGLEADADDS NTRSLLLSTK QLLADIIQFH PGDTLKEILS LSASREQEAA HKQLMSRRQA CTAQTPEPLR RHRSLTAHSL LPLAEKQRRV LRNLRRLEAL GLVSARNGYQ GLVDELAKDI RNQHRHRHRR KAELVKLQAT LQGLSTKTTF YEEQGDYYSQ YIRACLDHLA PDSKSSGKGK KQPSLHYTAA QLLEKGVLVE IEDLPASHFR NVIFDITPGD EAGKFEVNAK FLGVDMERFQ LHYQDLLQLQ YEGVAVMKLF NKAKVNVNLL IFLLNKKFLR K 1631

FI using IQGAP-1 Antibody	FI using IQGAP-1 , C-Terminal Antibody	WB using IQGAP-1 Antibody

using IQGAP-2 (519-727) Antibody

Antibody Name	Application	Specices	Host	Catalog No.:	Size	Price
IQGAP-1 , C-Terminal Antibody	FI, WB, E	h	goat	PS001-36-AG-01	50 ug	160
IQGAP-1 Antibody	WB	h	goat	PS001-36-AG-02	50 ug	320
IQGAP-1 Antibody	FI, WB, E	h, m, r	goat	PS001-36-AG-03	50 ug	160
IQGAP-2 (519-727) Antibody	FI, WB	h	goat	PS001-36-AG-06	50 ug	160
IQGAP-2 Antibody	WB	h	goat	PS001-36-AG-07	50 ug	320
IQGAP3 Monoclonal Antibody	WB, E	h	mouse	PS001-36-AG-09	50 ug	290

Proteomic analysis of CTGF-activated lung fibroblasts: identification of IQGAP1 as a key player in lung fibroblast migration

Connective tissue growth factor (CTGF) is over expressed in lung fibroblasts isolated from patients with interstitial lung disease (ILD) and systemic sclerosis (SSc, scleroderma) and is considered to be a molecular marker of fibrosis. To understand the significance of elevated CTGF we investigated the changes in lung fibroblast proteome in response to CTGF overexpression. Using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometric analysis, we identified 13 proteins affected by CTGF. Several of the CTGF-induced proteins, such as pro-alpha (I) collagen and cytoskeletal proteins vinculin, moesin and ezrin, are known to be elevated in pulmonary fibrosis, whereas nine of 13 proteins have not been studied in pulmonary fibrosis and are, therefore, novel CTGF-responsive molecules which may have important roles in ILD. Our study demonstrates that one of the novel CTGF-induced proteins, IQ motif containing GTPase activating protein (IQGAP)1, is elevated in lung fibroblasts isolated from scleroderma patients with ILD. IQGAP1 is a scaffold protein that plays a pivotal role in regulating migration of endothelial and epithelial cells. Lung fibroblasts treated with CTGF demonstrated increased rate of migration in a wound healing assay. Depletion of IQGAP1 expression by small interfering (si)RNA inhibited CTGF-induced migration and Erk1/2 phosphorylation in lung fibroblasts. MAPK inhibitor decreased CTGF-induced cell migration and did not interfere with IQGAP1 expression suggesting that MAPK pathway is downstream of IQGAP1. These findings implicate the importance of CTGF in lung tissue repair and fibrosis and propose that CTGF-induced migration of lung fibroblasts is mediated via IQGAP1 and MAPK signaling pathways. Key words: scleroderma, lung fibrosis, CTGF, IQGAP1, cell migration.

Bogatkevich GS, et al. Am J Physiol Lung Cell Mol Physiol. 2008 Aug 1.

IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade

Proliferation of epithelial cells must be spatiotemporally regulated to maintain the organization of epithelial sheets. Here we show that the IQGAP family, comprising IQGAP1, 2 and 3, underlies lateral cell-cell contacts of epithelial cells. Of the three proteins, IQGAP3 is unique in that its expression is specifically confined to proliferating cells. Knockdown of IQGAP3 in cultured epithelial cells caused inhibition of proliferation and ERK activity. When exogenously expressed in quiescent cells, IQGAP3 was capable of inducing cell-cycle re-entry, which was completely inhibited by the MEK inhibitor U0126. Thus, IQGAP3 is necessary and sufficient for driving cell proliferation and ERK acts downstream of IQGAP3. Furthermore, IQGAP3 specifically interacted with the active, GTP-bound form of Ras, and in IQGAP3 knockdown cells, the activity of Ras, but not of other small GTPases, was inhibited. Thus, IQGAP3 regulates the promotion of cell proliferation through Ras-dependent ERK activation.

Nojima H, et al.Nat Cell Biol. 2008 Aug;10(8):971-8. Epub 2008 Jul 6.

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Ras GTPase-activating-like protein IQGAP1, -2 & -3

Proteomic analysis of CTGF-activated lung fibroblasts: identification of IQGAP1 as a key player in lung fibroblast migration

IQGAP3 regulates cell proliferation through the Ras/ERK signalling cascade